Kinetics of Insulin Adsorption from Real Time of Measurements in a Microfluidic Chip
نویسندگان
چکیده
We propose an original method to monitor insulin adsorption by EOF mobility measurements. Kinetics have been real-time recorded by particle anemometry in a Wheastone microfluidic bridge. Hybrid glass/photosensitive PDMS/glass microfluidic chips were fabricated to perform mixed hydrodynamic and electrophoretic cycles, with different insulin concentrations. From the real-time measurements the constants of adsorption kinetics were determined by fitting the extracted data according the Langmuir model. This platform offers accurate results even at 10 M insulin concentration.
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